LOCUS       40924_23514       11576 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB627 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11576)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11576)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1210
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     protein_bind    1220..1344
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1381..1411
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1465..2121
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2466..2768
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2812..2936)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2962..3214
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3248..3264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3272..3288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3296..3326)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3341..3362)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3398..3650)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3813..4361)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4413..4592)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5098..5122)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5643..6431
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6680..7268
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7454..7594)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7938..8132)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8201..9271)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9703..10329)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gctagcatga ctggtggaca
     1201 gcaaatgggt gctgttatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
     1261 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     1321 aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg caccccaggc
     1381 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     1441 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     1501 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1561 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1621 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1681 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1741 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1801 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1861 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     1921 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1981 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     2041 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     2101 gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag
     2161 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     2221 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     2281 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     2341 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     2401 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     2461 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     2521 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     2581 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     2641 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     2701 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     2761 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     2821 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     2881 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgata
     2941 acctagagct cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg
     3001 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     3061 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     3121 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     3181 ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tggttccgga accaattcgt
     3241 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3301 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3361 taggctgaat taggcgcgcc tatttctatt aattcccgat ctagtaacat agatgacacc
     3421 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     3481 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     3541 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     3601 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
     3661 gagctgggga cgtccccagc tcggtacccc ctcctgggca cggtcgtacg cgatccgccg
     3721 cggttccacc tcaccattgc gggtatgcca gttgagttcg atgagttccc gtaccagctc
     3781 caactcgggc gatgccacgc gcactccgtt cagatctcgg tgacgggcag gaccggacgg
     3841 ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc
     3901 ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc
     3961 acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc
     4021 agggacttca gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg
     4081 gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc
     4141 gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc
     4201 cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg
     4261 accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg
     4321 gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tatcttattg ccccccggga
     4381 tcagatctgg attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga
     4441 acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact
     4501 tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc
     4561 gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt
     4621 cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcttgat
     4681 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
     4741 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
     4801 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
     4861 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     4921 tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg
     4981 cggcagcgtg aagctgatcc gtcgagatta atagaaataa attcagccta attcggcgtt
     5041 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     5101 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     5161 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     5221 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     5281 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     5341 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc
     5401 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     5461 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     5521 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     5581 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     5641 tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     5701 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     5761 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     5821 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     5881 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     5941 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     6001 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     6061 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     6121 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     6181 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     6241 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     6301 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     6361 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     6421 tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg
     6481 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     6541 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat
     6601 catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6661 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6721 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6781 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6841 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6901 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6961 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7021 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7081 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7141 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7201 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7261 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7321 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7381 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7441 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7501 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     7561 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     7621 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     7681 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc
     7741 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     7801 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     7861 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     7921 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     7981 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     8041 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     8101 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc
     8161 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     8221 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     8281 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     8341 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     8401 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
     8461 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
     8521 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
     8581 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
     8641 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
     8701 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
     8761 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
     8821 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
     8881 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
     8941 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
     9001 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
     9061 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
     9121 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
     9181 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
     9241 gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt
     9301 gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc
     9361 acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc
     9421 tttagccgct aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg
     9481 cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca
     9541 gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg
     9601 ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg
     9661 tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat
     9721 ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac
     9781 ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag
     9841 aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt
     9901 gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg
     9961 cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg
    10021 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
    10081 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
    10141 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
    10201 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
    10261 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
    10321 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
    10381 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
    10441 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
    10501 aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc
    10561 gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg
    10621 tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct
    10681 cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct
    10741 cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct
    10801 ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg
    10861 tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc
    10921 gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca
    10981 ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg
    11041 cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc
    11101 ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca
    11161 gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg
    11221 ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat
    11281 agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc
    11341 aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg
    11401 cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca
    11461 ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat
    11521 cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat aaacct
//
